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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Expression of the Clustered NeuAcα2–3Galβ O -Glycan Determines the Cell Differentiation State of the Cells
doi: 10.1074/jbc.M114.550848
Figure Lengend Snippet: MAb7 marks undifferentiated hESCs. A and B, undifferentiated hESCs (image; left, immunostaining; right) or cryosections of SFEBq-cultured hESC aggregates on days 0, 2, 3, and 7 were immunostained with Pax-6 (red), Hoechst 33342 (blue), and MAb7 (green) (A) or TRA-1–60 (green) (B). White bars, 100 μm. C, 10 μg of lysates from SFEBq-cultured hESC aggregates on days 0, 2, 3, and 7 were prepared. The expression levels of MAb7 antigen, TRA-1–60 antigen, and Pax-6 were determined in the same Western blot membrane. D, human amniotic epithelial cells were immunostained with Hoechst 33342 (blue) and MAb7 (red) or TRA-1–60 (red). White bars, 100 μm.
Article Snippet:
Techniques: Immunostaining, Cell Culture, Expressing, Western Blot, Membrane